The Proteintech Blog

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Mitochondria are more than the Powerhouse of the Cell

admin July 20, 2016

  By Sophie Quick Introduction to mitochondria Mitochondria are the result of a billion-year old fusion of primitive bacteria and early eukaryotic cells, resulting in a species-defining endosymbiosis. These organelles not only generate the cell’s chemical energy through ATP-proton pumps but have emerged as key players in a number of cellular processes including signalling, differentiation… Continue Reading »

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Mitochondria: Center of cellular signaling & energetic balance

admin June 27, 2016

Mitochondrial markers The mitochondrion is composed of an inner and outer membrane, intermembrane space, cristae, and matrix, and contains its own DNA separated from the nuclear DNA. Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Colocalization with one of the organelle-specific markers… Continue Reading »

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10 tips on how to optimize siRNA transfection

admin June 21, 2016

siRNA transfection is a powerful tool used to understand the mechanisms of gene regulation and different molecular pathways. How does it work? Transfecting siRNA with a high efficiency while avoiding side effects is influenced by several different factors. The following 10 tips will help you to optimize your siRNA transfection. 1. Protocol optimization The following… Continue Reading »

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Introducing New Career Development Podcast: The Incubator with Dr. Olds

admin June 16, 2016

Fret over your scientific career no longer. Proteintech is sponsoring a monthly career development podcast, The Incubator, on June 23, for graduate students and postdocs interested in careers beyond academia. Dr. William Olds will be interviewing scientists who have gone into careers ranging from industry to the government, probing their minds for how to enter… Continue Reading »

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Story of CA IX (11443-1-AP)

admin June 14, 2016

What are CAs? The carbonic anhydrases (CAs; EC 4.2.1.1) are a family of metalloenzymes that catalyze the reversible hydration of carbon dioxide and bicarbonate. Among those identified, there are eight cytosolic proteins (CA I, CA II, CA III, CA VII, CA VIII, CA X, CA XI, CA XIII), two mitochondrial matrix proteins (CA VA, CA VB), one secreted protein… Continue Reading »

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Nanodiamonds and cancer

admin April 27, 2016

Introduction to nanoparticles Recently, expressions such as nanomedicine, nanoparticles, or nanomaterial have grown very popular amongst the scientific community. What is so exceptional about ‘nano’ research? Indeed, it is simply the small size; a nanoparticle is small enough to enter a live cell, but still has the potential to carry a substantial therapeutic payload or… Continue Reading »

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Molecular markers for liver cancer

admin March 24, 2016

Early HCC diagnostic is still missing Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), primary liver cancers, are the fifth most common cancers and the third most common cause of cancer mortality (Parkin DM, 2000). Gold standards for cancer diagnostics include classification and subtyping of cancer-based on imaging, serum markers, tissue analysis and molecular analysis of markers… Continue Reading »

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Fast Phospho-rward: Making Phosphoprotein Antibodies

admin January 4, 2016

By William Olds, PhD Cells are packed to the brim with protein, lipids, carbohydrates, nucleic acids, and others. To make sure all these substances work together to sustain life, they need to be regulated. Phosphorylation is a key way that cells toggle activity of their equipment, playing integral roles in processes spanning from embryonic development… Continue Reading »

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Recalling When and Where | How the Brain Processes Episodic Memory

admin November 6, 2015

  By Deborah Grainger Neuroscientists based at MIT have recently identified a novel brain circuit that processes the ‘where’ aspect of memory. Pairing this with their previous work on how the brain remembers the ‘when’ component, we now possess a greater understanding of memory construction. When you remember an event, you reassemble several separate pieces of… Continue Reading »

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Tech Tips | In search of low molecular weight proteins

admin August 26, 2015

  By Deborah Grainger Regular SDS-PAGE and Western blotting techniques are robust tools for investigating a broad spectrum of proteins with molecular weights (MWs) ranging from about 30 kilodaltons (kDa) up to 250 kDa. However, at extreme ends of the MW spectrum such techniques suffer from limitations: poor separation, signal reduction or even a total absence… Continue Reading »

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