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Antibodies for Stress Granule Research

admin January 10, 2014

 

Immunofluorescence image showing no "granulation" under conditions of no stress and typical "granulation" under conditions of cell stress using Proteintech's YB1 antibody (red).  The smaller green bodies are processing bodies (P-bodies), which  localize with stress granules though have their own discreet functions.

Immunofluorescence image showing no “granulation” under conditions of no stress and typical “granulation” under conditions of cell stress with arsenite. Stress granules have been labelled using Proteintech’s YB1 antibody (red). The smaller green bodies are processing bodies (P-bodies), which localize with stress granules though have their own discreet functions. Credit: Nancy Kedersha. (Click the image to see a larger version.)

 

Eukaryotic cells have evolved sophisticated strategies to combat a barrage of cellular stresses. Under stress, a cell’s main priority is to conserve energy and divert the rest toward survival, and where possible, recovery. Stress granules help by limiting protein production to just the essential proteins needed for survival. They act as foci of molecular triage, sorting untranslated messenger RNAs (mRNAs) into those that absolutely must go on to make proteins, those that should stay under “protection” and those the cell can do away with. Upon receiving a stress signal, for example in response to heat shock, chemical exposures, glucose deprivation or oxidative stress, any mRNAs yet to undergo translation, along with their chaperone proteins, are aggregated and assembled into stress granules. This process is complex and is known to involve over 100 different proteins – although, it is difficult to determine their exact function in stress granule formation as they have other roles elsewhere in the cell. Moreover, different proteins are recruited to stress granules depending on the type of stress and some may only stay around for a short time, even seconds, before moving on.

Read Imaging a Hidden Universe | A Story of Stress Granules for more on this subject.

In summary, this complex field of research has wide applicability to many cell processes and signals, and aspects of stress granule investigation can be found in the study of apoptosis, neurodegeneration, cancer and immunology as well as in many other areas.

Check out a sample of the antibodies Proteintech has available for Stress Granule research below:

Antibody Catalog Number Tested Applications Tested for SG localization by an independent party
ATXN2 21776-1-AP ELISA, WB, IHC, IF                    Yes
Caprin1 15112-1-AP ELISA, WB, IHC, IP                    Yes
eIF2C2 10686-1-AP ELISA, WB
eIF4G1 15704-1-AP ELISA, WB, IHC, IF                    Yes
EIF4G2 (DAP5) 17728-1-AP ELISA, WB                    Yes
FXR1 13194-1-AP ELISA, WB, IHC, IP, IF
G3BP-1 13057-2-AP ELISA, WB, IHC, IP, IF                    Yes
Musashi-2 10770-1-AP ELISA, WB, IHC
STAUFEN1 14225-1-AP ELISA, WB
TARDBP (TDP-43) 10782-2-AP ELISA, WB, IHC, IP, IF                    Yes
USP10 19374-1-AP ELISA, WB, IF                    Yes
YBX1 (YB1) 20339-1-AP ELISA, WB, IHC                    Yes

Key: SG= Stress granule, WB = Western blotting, IHC = immunohistochemistry, IP =immunoprecipitation, IF = immunofluorescence

 

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