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Antibodies for Kidney Research

admin July 11, 2013

After attending NephroTool’s first International Conference, held at the University of Liverpool this month, it seemed particularly relevant to dedicate a post to kidney research. We offer many different antibodies for this research area; some are detailed in the post below, which focuses on several successes in kidney-related research featuring our antibodies.

COL1A2

Proteintech’s anti-COL1A2 antibody (14695-1-AP) has featured in an Aging Cell paper exploring molecular events associated with aging-induced matrix changes in the kidney. The study, following C57BL/6 mice throughout youth, middle and old age, observed increases in the content of collagen types I and III and fibronectin in the kidney matrix as the mice grew older. This was accompanied by albuminuria and elevation of cystatin C indicating decreasing glomerular filtration, associated with the activation of the TGFβ/SMAD3 canonical signaling pathway. Aging-specific, collagen-related changes in the kidney saw decreased levels of ZEB1 and ZEB2 – collagen type 1 transcription inhibitors – and their binding to the collagen type 1α2 promoter, perhaps underlying the increases in latter’s gene product. The Proteintech COL1A2 antibody was used in the study to assess protein levels of collagen type 1α2 by immunoblotting.

Inversin

Immunofluorescence staining of human TERT-RPE1 cell cilium. Proteintech anti-inversin antibody (INVS/NPHP2; catalog no. 10585-1-AP) marks the ‘inversin compartment’ and basal body of the cell (green). Courtesy of Dr. Kevin Corbit.

Immunofluorescence staining of human TERT-RPE1 cell cilium. Proteintech anti-inversin antibody (INVS/NPHP2; catalog no. 10585-1-AP) marks the ‘inversin compartment’ and basal body of the cell (green). Courtesy of Dr. Kevin Corbit.

Whilst on the subject of the aging kidney, Proteintech’s anti-inversin antibody (10585-1-AP) appeared in another aging-related study investigating the pathological changes of hypertensive renal disease in aged, spontaneously hypertensive rats. Hypertension is a major cause of chronic renal failure and leads to afferent arteriolopathy and glomerular collapse. The group observed that these hypertension-induced events lead to tubular atrophy (a reduction in tubular diameter), which possibly results in reduced urinary flow through affected tubules. Supporting this, chronically damaged tubules in the aged rats showed reduced staining for inversin using Proteintech’s inversin antibody. Inversin is a ciliary protein essential for kidney development and function, and reduction in its levels was indicative of changes in ciliary signaling linked to reduced urinary flow.

SDCCAG8

Cilia provide a link to the next kidney-related research paper in this selection of articles featuring Proteintech antibodies. The antibody of focus targets SDCCAG8, and was used in a paper examining ciliopathy candidate genes and their involvement in nephronophthisis-related ciliopathies (NPHP-RC). NPHP-RCs are single-gene recessive disorders, which cause retinal-renal ciliopathies that affect the kidneys, retinas, brain and liver. The authors of the Nature Genetics paper identified 12 different truncating mutations in SDCCAG8 in 10 NPHP-RC families, expanding the cohort of known NPHP-RC-causing genes from the 9 described previously. Proteintech’s anti-SDCCAG8 antibody (13471-1-AP) was employed for more in-depth analyses of SDCCAG8’s role in NPHP-RC using a variety of experimental techniques including: co‑immunoprecipitation, immunofluorescence and its immunogold labeling for staining of ultrathin mouse retinal sections.  

Don’t just take our word for it, listen to a satisfied customer:

Six2 positive cells (red) in condense metanephricmesenchyme surrounding the ureteric bud tip. Immuofluorescence staining of mouse embryonic kidney rudiment dissected at E13.5, cultured for 2 days and fixed in methanol, using Six2

Six2 positive cells (red) in condense metanephricmesenchyme surrounding the ureteric bud tip. Immuofluorescence staining of mouse embryonic kidney rudiment dissected at E13.5, cultured for 2 days and fixed in methanol, using Six2

Long-time Proteintech antibody user Alexsandra Rak-Raszewska from the University of Liverpool, whose Organogenesis paper “Development of embryonic stem cells in recombinant kidneys” featured Proteintech’s anti-Six2 , has had great success with our antibodies in the past. She had this to say about our Six2 antibody:

 “The Six2 antibody works great and is really specific to metanephric mesenchyme (kidney progenitor cells). We used it for immunofluorescence and we highly recommend it!”

Anti-Six2 can be seen staining the metanephric mesenchyme in Alexsandra’s immunofluorescence results on the left.

If you would like to be a Proteintech success story like Alexsandra, then we offer an array of high-quality antibodies to support your kidney research. Please visit www.ptglab.com for our full list of antibodies targeting over 10,000 human proteins – fully guaranteed to work in your experiments or your money back. 


 

 

 

 

 

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