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actin

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Western Blot Loading Control Antibodies

admin August 3, 2012

by Deborah Grainger+   The proteins and peptides that regularly serve as endogenous or internal controls may not always take center stage in your research, but they are indispensable to your conducting meaningful experiments and are essential for publication. The Western blot is an immunoassay that requires such controls as they are widely used for determining specific… Continue Reading »

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Loading control antibodies from Proteintech

admin July 8, 2010

Loading controls are used to determine whether samples have been loaded equally across a gel. They also serve as internal positive controls in determining that all reagents are functioning properly and to assess the transfer of protein during western blotting. They are normally general cellular proteins but more specific markers are sometimes used for subcellular… Continue Reading »

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ACTB and the actin family

admin June 16, 2010

Actins are a family of highly conserved cytoskeletal proteins that are involved in cell motility, structure, and integrity. Vertebrates have six actin isoforms. Two are found in striated muscle (α-skeletal and α-cardiac), two in smooth muscle (α- and γ-SMA) and two are cytoplasmic (β-CYA and γ-CYA, called ACTB and ACTG1 respectively). Muscle actins are tissue… Continue Reading »

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