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The proteins that normally serve as internal controls in your research may not always take center stage, but they are indispensable for conducting meaningful research, most notably for the proper interpretation of Western blots.
Loading control proteins and protein tags provide a reference to evaluate the changes in expression levels of target proteins and help to ensure that any increase or decrease in target protein levels is due to your experimental manipulations, and not due to errors in the Western blot procedure.
Proteintech control antibodies are $99 each (€149 in Europe) for a 150ul size vial.
|Alpha Tubulin||66031-1-Ig||Mouse Mono||ELISA, WB, IHC, IF, FC||28|
|Beta Tubulin||66240-1-Ig||Mouse Mono||ELISA, WB, IHC, IF, IP||3|
|Gamma tubulin||66320-1-Ig||Mouse Mono||ELISA, WB, IF|
|Beta Actin||66009-1-ig||Mouse Mono||ELISA, IHC, WB, FC, IP, IF||118|
|GAPDH||60004-1-Ig||Mouse Mono||ELISA, WB, IHC, IF, IP, FC||367|
|6 HIS-TAG||66005-1-Ig||Mouse Mono||ELISA, WB, IF, IP||60|
|FLAG-TAG||66008-2-Ig||Mouse Mono||ELISA, WB||12|
|GFP-TAG||66002-1-Ig||Mouse Mono||ELISA, WB, IF, IP||44|
|MBP-TAG||66003-1-Ig||Mouse Mono||ELISA, WB, IP||1|
|MYC - TAG||60003-2-Ig||Mouse Mono||ELISA, IF, IP, WB||7|
|V5-TAG||66007-1-Ig||Mouse Mono||ELISA, WB, IP||3|
|Vinculin||66305-1-Ig||Mouse Mono||ELISA, WB, IF, IHC, FC||1|
|TBP||66166-1-Ig||Mouse Mono||ELISA, WB, IP, IHC, IF||1|
|Catalog number: 60004-1-Ig|
This enzyme is commonly used as a protein loading control in Western blotting due to it's consistently high expression in most cell types. GAPDH participates in several cellular events such as glycolysis, DNA repair and apoptosis.
|Catalog number: 66009-1-IG|
Actins provide structural integrity and mediate contraction, motility and trafficking in cells. Beta-actin is a non-muscle, cytoskeletal actin isoform, expressed in a uniform manner in most cell types, making it a good internal control in Western blotting experiments.
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